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![]() ![]() ![]() The A shows that the IME is in "Alphabet" mode. You should see the Japanese IME icons just to the left of the clock. Optionally, click the blue Language bar options or Input language hot keys to configure those options as well.Under Switching input methods, you should probably leave these unchecked, unless you have other IMEs installed (perhaps different Japanese IMEs, or even IMEs for other languages like Korean or Chinese).Under Override for default input method, open the drop-down and select Japanese - Microsoft IME.Now click Keyboard to change this to the Japanese IME. ![]() Also make sure that Japanese is shown in the list under Preferred languages, and that Japanese appears u̲n̲d̲e̲r̲ your preferred variety of English. Make sure that the Windows display language is still set to your preferred flavor of English. You should be back at the Language settings dialog.Make sure to U̲N̲C̲H̲E̲C̲K̲ the boxes for Install language pack (this installs all the language resources to display Japanese UI, which uses up a chunk of drive space and which you don't need), and for Set as my Windows display language (since you want to keep English for your UI display). For Install language features, the Preferred language will show only 日本語, so leave that as-is.For Choose a language to install, type japanese to shorten the list, select Japanese, and click Next.Under Preferred languages, click Add a language.It is possible that this might differ for someone using a consumer version - on Windows XP, for instance, the language resources were not included in the default install, and required a separate installation disk or downloading first. This is a walkthrough of the UI using a corporate installation of Windows 10. ![]() ![]() ![]() On the heels of my interview with Adrian Bird (published in the October issue of PLoS Genetics), I made my way to Jeffreys through another branch of the British Rail system. Like Jeffreys himself, I wanted to dig below the surface of this discovery as well as that of another genetic nugget-the intervening sequence-found as a post-doctoral fellow seven years earlier. To use Jeffreys' words, he has been “branded” by DNA fingerprinting, but he delights in its application and the hook it provides for public curiosity about science. TaqI is a restriction enzyme from the same type of organism that produces Taq DNA polymerase (Thermophilus aquaticus or Thermus aquaticus).In 1984, while tracking the veins of globin gene evolution and panning the human genome for hypervariable linkage markers, Sir Alec Jeffreys accidentally struck gold-he discovered a way to identify any human being by a DNA “fingerprint”. However, there are exceptional temparatures: SmaI (25C),ApaI (30C), BclI (50C), BstEII (60C) and TaqI (65C). Most restriction enzymes are used at 37C. RE Digestion What does a restriction enzyme need in order to do its duty? - a double-stranded DNA sequence containing the recognition sequence.- suitable conditions for digestion. What type of ends produced by the REs? RE Specific Sequence (5’ 3’) Type of ends SalI HaeIII HhaI HpaI MboI NotI PstI XhoI XbaI SacI Tutorial Give the specific sequences recognized by the REs. They may have different sites of specific cleavage.Restriction enzymes that recognize the same sequence and are derived from different organisms.REs 4-base cutters 6-base cutters HpaII CCGG HindIII AAGCTT S S RsaI GTAC EcoRI GAATTC B S TaqI TCGA DraI TTTAAA S B AluI AGCT BamHI GGATCC B S HinfI GANTCěglI AGATCT S S DdeI CTNAGĜlaI ATCGAT S S B = blunt ends S = sticky ends (overhang) Sticky ends 5’G/CGC3’ 5’G CGC3’ CfoI 3’GCG/C5’ 3’CGC G5’ 5’GAT/ATC3’ 5’GAT ATC3’ Blunt ends EcoRV 3’CTA/TAG5’ 3’CTA TAG5’ Sticky ends 5’G/CGC3’ 5’G CGC3’ CfoI 3’GCG/C5’ 3’CGC G5’ 5’GAT/ATC3’ 5’GAT ATC3’ Blunt ends EcoRV 3’CTA/TAG5’ 3’CTA TAG5’ REs 4-base cutters 6-base cutters HpaII CCGG HindIII AAGCTT RsaI GTAC EcoRI GAATTC TaqI TCGA DraI TTTAAA AluI AGCT BamHI GGATCC HinfI GANTCěglI AGATCT DdeI CTNAGĜlaI ATCGAT Some REs produce blunt ends and others produce sticky ends. 4-base cutters 6-base cutters HpaII CCGG HindIII AAGCTT RsaI GTAC EcoRI GAATTC TaqI TCGA DraI TTTAAA AluI AGCT BamHI GGATCC HinfI GANTCěglI AGATCT DdeI CTNAGĜlaI ATCGAT over 3000 REs have been studied in detail, and more than 600 of these are available commercially.Two catalytic manganese ions (one from each monomer) are shown as magenta spheres and are adjacent to the cleaved sites in the DNA made by the enzyme (depicted as gaps in the DNA backbone). Structure of the homodimeric restriction enzyme EcoRI(cyan and green cartoon diagram) bound to double stranded DNA (brown tubes). BamHI (Bacillus amyloliquefaciens) HindIII (Haemophilus influenzae) TaqI (Thermus aquaticus).Iğirst identified (order identified in bacterium).Restriction enzymes are named based on bacteria in which they are isolated in the following manner:.Daniel Nathans and Hamilton Smith received Nobel Prize in Medicine (1978) for the discovery of restriction endonucleases, leading to the development of recombinant DNA technology.DNA genome of pine tree restricted by EcoRI can generate 5 million different restricted fragments.RE acts as molecular scissors to cut DNA molecules at specific sequence.RFLP was developed at the late 70’s due to the discovery of restriction enzymes (REs or called as restriction endonucleases) from bacteria.RFLP(Restriction Fragment Length Polymorphism) ![]() ![]() ![]() To be fair, the article never exactly says the fonts are racist or objectively offensive, though it does have this classic junk: Musing about this article, Matt Margolies says, “The question of whether fonts can actually be racist is obviously a sign that true, legitimate racism is so hard to find that we actually have to make everything inherently racist just to have something to complain about.” Well, yes, except that the Left is in the process of re-defining racism to mean “anything said or done in America or by Americans, unless it is said or done by an African American or another Perpetual Victim of Color (PVC).”Īnn Quito, the author, argues that type fonts that attempt to evoke or reference another culture or locale like this…Īnd, though she doesn’t mention it, this (African)…Īnd if anybody cared about what they thought, this… If the author does believe it, he or she (it’s a she this time) is obviously a victim of the dreaded “political correctness ate my brain” pandemic, so why pay any more attention to what she writes about fonts than if she had ranted on about Republican beetle larvae burrowing into her brain? So when it comes out with something like this idiocy, complaining about “racist fonts,” what is the ethical response? It’s click-bait, so there is no way to determine whether anyone, even the author, believes what the article says. It also raises the question, “How does one correctly regard what CNN says and publishes?” We know now that the network cares not a whit about journalism, nor facts, nor objective analysis, not integrity. It’s like a return to the 1950s.This dispatch from The Great Stupid raises the question, “How stupid can the Great Stupid get before a critical mass of the public says, “Hey! This stuff is stupid!“? But we will leave that one for another day. ![]() ![]() However, Yang points out that at the bottom of the cited Design Observer article, the writer notes, "Granted, unlike people, typefaces have no feelings - so who cares if they’re used without sensitivity and knowledge? But on some level, the line is a murky one: What’s the difference between a celebrity making an unforgivable racist remark and a typographer making a font that clumsily perpetuates a cultural stereotype?"Īnd Paul Shaw, design historian and School of Visual Arts professor, told Yang that the chop suey and chopsticks styled letters are usually linked to unflattering stereotypes, "You’ll see caricatures with slanted eyes and buck teeth." He added, "You’d think FreshDirect would know better. Reached this week, FreshDirect PR manager Maria Coder added that “Our team gave this typeface a lot of thought before integrating it into our packaging and advertising.” Likewise, nobody questions a sign for a burrito restaurant designed in Hot Tamale, but what about when it’s used for a border crossing sign in Texas?’ We agreed with this evaluation of ‘appropriate use.’” “ says, ‘Clearly, some fonts are just too Western, and that’s where modification…can, when done well, can be extremely useful….And yes, it’s all about appropriateness: fine to use Fake Hebrew for a deli not so fine on, say, a yellow armband. Interestingly, the spokesperson then went on to quote an article in Design Observer that the FreshDirect design team read and debated before deciding to go ahead with the chop suey/chopstick font choice. The FreshDirect creative team did not in any way intend to offend anyone with the use of the typography, which was meant to echo a classic typeface found in many menus and takeout boxes. In response, a company spokesperson sent back an email that read, in part: “Thank you for taking the time to express your thoughts and share your concerns. Yang's friend had emailed FreshDirect to complain about their "unfortunate use of stereotypical typography" and how she was “disappointed by the laziness of using an outdated and unimaginative font in the logos to advertise the ‘Orientalness’ of the cuisine." From Yang: ![]() And, apparently in an attempt to make it more authentically Asian, "Stir Fry Kits" is written in what Yang calls that "cliche fake-brushstroke 'oriental' typeface that reflexively causes many Asians to cringe," which is called "chop suey lettering" while "Dumplings" is written in what's known as "chopsticks lettering." , who writes the Tao Jones columnist for the Wall Street Journal Online, observed earlier this week that FreshDirect sent an email promoting a new line of "quick and easy" food options, Stir Fry Kits. ![]() ![]() ![]()
![]() ![]() Outside food & beverages are not allowed except for one (1) sealed water bottle and one (1) clear gallon-sized bag for snacks. Please check local traffic reports for real time updates. Early arrival and carpooling are strongly recommended. Limited quantities are available.ĭue to event and local traffic, please expect delays and give yourself plenty of time to arrive at Jiffy Lube Live. Preferred parking options and parking for oversized vehicles may be purchased in advance here. General admission lot parking is included with your ticket purchase. We will allow clear plastic, vinyl, or PVC tote bags no larger than 12” x 6” x 12” and/or small clutch bags no larger then 6”x 9”. Official Race WebsiteĮver run in the Prince William Half Marathon? Share your impressions here, as well as anything you learned about the race that you think other runners/walkers should know.Please note our Clear Bag Policy will be enforced, we are a Cashless venue and in most cases your ticket is on your phone. To reserve your spot in the 2019 running of Bristow’s Prince William Half Marathon, register online at here. See the race route map for the Prince William Half Marathon here.
![]() It would appear that this disruption of the normal growth pattern is a response to a parasitic organism. ![]() An imbalance of the hormones in the plant can stimulate these extra buds and produce an abnormal congested growth. These buds are typically held in reserve in case of injury to the plant. It is well known that every branch of a woody plant produces many more bud initials than needed for normal development of that plant. The Sargent’s weeping hemlock, for example, can be raised from seed and tends to produce offspring identical to the dwarf plant. The progeny from such seeds vary widely, but some seem stable. Occasionally, the broom will develop cones and set viable seed. Other dwarfs are not as stable and must be constantly policed for aggressive growth patterns that are the plant’s attempt to revert to its natural state. Successful experimentation, however, has produced some relatively stable dwarfs such as the well-known “bird’s nest” or dwarf Alberta spruce. Propagation is difficult, as both grafts and cuttings tend to have a high mortality rate. Both asexual and sexual propagation of the brooms, when successful, tend to produce a dwarf plant having the basic characteristics of its normal sized parent but usually with a much denser growth pattern. Most of the dwarf conifers that are becoming so popular today are the progeny of witches’ brooms. ![]() It was once believed that witches flying low over certain trees produced these “brooms.” After talking to experts at two Canadian schools, I favor this medieval interpretation – but will leave it to your judgment. ![]() Witches’ brooms are dense congested growths appearing on the lateral branches of many woody plants. Since the abnormal growth tends to be upright, the result gives the impression of a small broom, growing on a normal sized tree. ![]() ![]() ![]() It didn’t matter that the movie featured gorgeous aesthetics and hugely impressive action set-pieces. Box office returns of $264,000,000 were considered a huge disappointment for a movie that cost somewhere in the region of $175,000,000, killing the mainstream career of director Kevin Reynolds and plunging Oscar-winning megastar Costner into frosty commercial waters. Starring Kevin Costner as a Maxian loner who fit a very familiar profile, Waterworld was written off, somewhat unfairly, as Mad Max on water, thanks to a thinly veiled, like-for-like premise and a cast of characters who looked like they’d jet skied their way off the set of The Road Warrior. Perhaps the most famous example of a Mad Max derivative happened to be the most expensive movie ever made back in 1995. Mad Max wannabees weren’t restricted to the B-movie doldrums. A young Patrick Swayze even tried Max on for size in 1987’s Steel Dawn, the same year Roddy Piper starred in the Miller-influenced, post-apocalyptic sci-fi comedy Hell Comes to Frogtown. In the US, low-budget opportunists Charles Band and Golan-Globus jumped on the Mad Max bandwagon with movies such as Metal Storm: The Destruction of Jared-Syn (1983) and Jean-Claude Van Damme action vehicle Cyborg (1989). Aussie neighbours New Zealand gave us the rather familiar Battle Truck (1982), the title of which should tell you all you need to know about that particular movie. The film’s Ozploitation roots provided the perfect platform for Italian genre cinema, scuzzy efforts 1990: The Bronx Warriors (1982), Escape from the Bronx (1983), and The New Barbarians (1983) straight out of the Mad Max playbook. All you needed was a desolate landscape, a few broken-down vehicles and some rag-tag outfits and you could pass yourself off as one of the most imitated movies of its generation, of any generation, at least until someone yelled “Action!” There was even a hugely popular pro wrestling tag team named the Road Warriors who dressed and acted exactly like the movie’s twisted metal villains, their name of course lifted from the film’s alternate title The Road Warrior, a supremely cool moniker created for American audiences who weren’t familiar with the original movie’s limited release.Ī slew of bargain-basement impostors crawled out of the nuclear dust in the wake of Mad Max 2‘s success, chiefly because, as proven by 1979’s hyper low-budget Mad Max, the concept could be done on the cheap. There were countless toy ranges and video games based on Miller’s creation. ![]() Even today George Miller’s ravaged vision of society is prevalent throughout the sci-fi genre, particularly its innovative world building and costume design. You only have to look at what preceded it and what followed to understand its influence. Not only is Mad Max 2 a perfect movie, it’s one of the most influential dystopian films ever made, becoming the standard bearer for not only action sequels, but action movies in general. Meet Max, “the Road Warrior” Max, in George Miller’s hugely innovative action sequel ![]() ![]() ![]() Written by Ed Sheeran for his wife-to-be Cherry Seaborn, this romantic, heart-felt ballad was rereleased as a duet with none other than 28-time Grammy winner Beyoncé. ![]() Streamed 1 billion times and counting on YouTube, this powerful tune co-written by guest vocalist Mikky Ekko is one of Rihanna’s best and will challenge even the most skilled singers to belt it out with pure emotion.įind Rihanna tickets | Find Mikky Ekko tickets Ed Sheeran With Beyoncé - “Perfect Duet” Singing this steamy duet about a couple that’s unable to resist the love between them will amp up the intensity of any date night. Seriously, who wouldn’t want to croon “Rock me mama like a wagon wheel”? Extra points for bringing along some friends to sing back-up harmonies and getting the entire crowd to join in at the end.įind Darius Rucker tickets Rihanna Featuring Mikky Ekko - “Stay” Originally written by Old Crow Medicine Show and Bob Dylan (yes, that Bob Dylan), its evocative images and simple chorus make it perfect for karaoke. This feel-good country ditty is the ideal sing-along song for when the drinks are flowing and spirits are high. That said, it’s perfect for singalongs in all of its doo-wop glory.įind Billy Joel tickets Darius Rucker - “Wagon Wheel” ![]() Arguably one of Joel’s most famouos songs that everyone knows the lyrics to, it chronicles the plight of a blue collar “downtown man” trying to woo a sophisticated uptown girl. People of a certain age will remember when Billy Joel’s “Uptown Girl” blared from every radio from the east to the west coast in 1983. Don’t forget to grab your bestie to be your hype man (or woman) and rap/sing Daddy Yankee’s parts.įind Luis Fonsi tickets | Find Daddy Yankee tickets Billy Joel - “Uptown Girl” The romantic pop-reggaeton ballad that soundtracked every wedding and house party around the world in 2017 is tailor-made for karaoke. It’s hard to think of a song that gets more people out on the dance floor than “Despacito.” Imagine the tropical shores of Puerto Rico as the opening steel-sting guitar notes waft through the air, hinting at the irresistible Latin rhythms to come. “If I Ain’t Got You” is another one of those challenging tunes that requires some serious vocal chops to do it justice, or you can just give it your best shot, sing off-key and have some fun with it.įind Alicia Keys tickets Luis Fonsi Featuring Daddy Yankee - “Despacito” Singing it properly takes some serious sass, attitude and vocal chops, or at least a few beers and a similar story you can relate to that’ll get you in the right frame of mind.įind Carrie Underwood tickets Alicia Keys - “If I Ain’t Got You”Īlicia Keys’ world-weary and melancholy tune is more fitting for an emotional catharsis than a party vibe, and that’s just fine there’s a time and a place for everything. ![]() Who doesn’t love a powerful revenge song? This multi-platinum hit that showed us a whole new side to Carrie Underwood is a fan favorite. Soaring choruses, an inspirational message and ’80s synths - what’s not to like? Not to mention you can pull your best friend or significant other up onstage with you to make this rocking duet pure magic.įind Starship tickets Carrie Underwood - “Before He Cheats” This feel-good power ballad has all the makings of the perfect karaoke song. Don a cowboy hat and an embroidered button-down shirt and add some air guitar licks to the guitar solo midway through the tune to really get the crowd riled up.įind Lynyrd Skynyrd tickets Starship - “Nothing’s Gonna Stop Us Now” Who doesn’t love this down home country-rock classic? “Sweet Home Alabama” thankfully doesn’t require tremendous vocal range, so anyone with the right attitude and a bit of spirit can rock the mic. Pro tip: Watch Usher’s “U Remind Me” video for some dance move inspiration.įind Usher tickets Lynyrd Skynyrd - “Sweet Home Alabama” Come ready with some impressive dance moves for the long instrumental mid-song breakdown. Telling the story of a sensitive man who can’t be with a woman because she reminds him of someone who broke his heart, “U Remind Me” is great for crooning and doing your best emotive Usher impression. This upbeat R&B tune is a real crowd-pleaser. ![]() ![]() ![]() One feature that’s particularly cool is the app’s ability to handle multiple video tracks. (The app comes with thousands of royalty-free, original soundtracks, sound effects and loops to help you bring your videos to life.) You can sync your video to the beat of your chosen soundtrack and add all the usual finishing touches, like titles and transitions. You can capture professional videos directly from the app with the built-in camera, and easily edit footage with drag and drop functionality. Adobe Premiere RushĪs you’d expect from an Adobe product, Premiere Rush has plenty of neat features. How much does it cost?įree, with in-app purchases. In some reviews, users reported that it can be glitchy at times. If we’re being critical, the app offers minimal control and no trimming option. The app also syncs footage to the beat of your soundtrack, and offers AI editing assistance. Take advantage of access to 100+ free songs, with Flashbacks and QuikStories create videos based on your latest footage. Quik is a great choice if you’re looking for a free app that’s packed with features. You can create HD 1080p or 720p videos in cinema, square or portrait formatting for social sharing and even save your draft projects for up to seven days.īest of all, it’s totally free to use. And with access to 100+ free songs, you can throw in the perfect soundtrack, soo! Not only can you trim, zoom and rotate photos and video clips, but you can also choose from 26 different themes and a range of fonts, filters and graphics. Quik also lets you add transitions and effects onto a maximum of 200 photos and video clips from your photo library or GoPro Plus. The app is super clever in the way it uses AI – it analyses your videos to detect colours and faces in order to frame your footage perfectly. The first of two video editing apps from GoPro, Quik promises ‘awesome edits made easy.’ And it’s fair to say it delivers. ![]() |
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